hoechst 33342 صبغ 2021 » result.name

PureBlu™ Hoechst 33342 Dye Bio-Rad.

04/07/41 · Hoechst 33342 Staining Dye Solution ab228551 is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, microplate, cuvette, and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission-tocontent curve. PureBlu Hoechst 33342 Dye is a highly pure formulation of Hoechst 33342 in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension. Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 in blue binding dsDNA. The excitation and emission wavelength of PureBlu Hoechst 33342 Dye when bound to double stranded DNA are shown in Fig 2. The Stokes shift between excitation and emission wavelengths is large making it an excellent choice for nuclear counterstaining in Immunofluorescence Microscopy, especially when. 27/01/36 · This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ Fluorescent Cell Imager for routine nuclear staining in fluorescence microscopy and cell imaging applications.

Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.Features of Hoechst 33342 Fluorescent Stain: Hoechst dyeblue fluorescent stain specific for DNA i.e., nuc. Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. Hoechst® 33342核酸染色は、一般的な細胞浸透性の核対比染色で、二本鎖DNAと結合すると青色蛍光を発します。 この色素はアポトーシス細胞における凝縮濃縮核の区別や、ブロモデオキシウリジンと組み合わせた細胞周期の研究に使用されることが多いです。. Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU.

Hoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP strategy, is detected at two emission wavelengths after UV excitation: Hoechst Red. Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution. Hoechst 33342 binds preferentially to adenine-thymine A-T regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in.

Bisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content. Hoechst 33342 and Hoechst 33258 are structurally similar dyes that perform comparably as nuclear counterstains. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are highly cell membrane permeant and widely used in cell cycle studies and as. Search results for hoechst 33342 at Sigma-Aldrich. Compare Products: Select up to 4 products. Please select more than one item to compare. Hoechst 33342 Staining Dye Solution ab228551 is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, microplate, cuvette, and flow cytometry applications. It can also be used to detect the contents. PureBlu Hoechst 33342 Nuclear Staining Dye is a highly pure formulation of Hoechst 33342 fluorescent dye Figure 1 packaged in a user-friendly format. Hoechst 33342 is a cell-permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT-rich DNA sequences.

Hoechst 33342 - an overview ScienceDirect Topics.

Hoechst 33342 Solution 20 mM.

08/08/30 · See more supporting information at. PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells - A Fast Approach to Staining Nuclei 02/07/25 · Exclusion of Hoechst 33342 dye is a characteristic common to stem cells, as well as chemotherapy-resistant cancer cells. Normally, these dye-excluding cells can be sorted from enzymatically dissociated tissues with a UV cell sorter/flow cytometer. UV-flow cytometry can be expensive, time-consuming and not readily available to all laboratories. Hoechst Stains 2 BrdU Quenching Hoechst 33342 and 33258 are quenched by BrdU.11,12 Fluorescence is enhanced upon binding to dsDNA at stretches of at least three AT base pairs, but no binding to stretches of two or more GC base pairs.

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